描述
Laser Capture Microdissection: Methods and Protocols
Murray, Graeme I. (University of Aberdeen, Aberdeen, UK)
Curran, Stephanie (University of Aberdeen, Aberdeen, UK)
Binding: Hardcover
Pub. Date: December 2004
Pages: 310
ISBN: 1588292606
Abstract:
Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.
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